U SRIJEDU, 27. svibnja 2026., s početkom u 9:00 sati, prof. Marián Masár održati će predavanje pod naslovom:
“Advances in Microchip Electrophoresis: From Device Design to Analytical Applications”
Prof. Masár upoznat će članove HKD-a sa svojim dosadašnjim radom u razvoju novih analitičkih metoda temeljenih na elektroforezi za analizu različitih bioloških, farmaceutskih i okolišnih uzoraka.
Predavanje će se održati u predavaonici br. 3.
Abstract
Advances in microchip electrophoresis: From device design to analytical applications
Marián Masár
Comenius University Bratislava, Faculty of Natural Sciences, Department of Analytical Chemistry, Mlynská dolina, Ilkovičova 6, SK-84215, Bratislava, Slovakia
marian.masar@uniba.sk
Progress across life sciences, environmental protection, food and energy sustainability, national security, and related fields is driving demand for robust, automated analytical instrumentation capable of rapid response to increasingly complex samples. In line with the principles of green analytical chemistry, minimizing reagent use, waste generation, and energy consumption, miniaturized separation techniques are gaining attention for their ability to meet these requirements. Among them, microchip electrophoresis (MCE) stands out as one of the simplest miniaturized separation platforms. It offers high separation efficiency, fast analysis, straightforward automation, low reagent consumption, reduced waste, and low operating costs. Despite these advantages, the analysis of complex biological, food, pharmaceutical, and environmental matrices often requires sample pretreatment and/or coupling with highly sensitive detection techniques. Microchips with coupled channels enable the integration of fundamental electrophoretic modes, such as zone electrophoresis (ZE) and isotachophoresis (ITP), into online, multidimensional workflows [1,2]. This architecture allows sample pretreatment and separation to be performed within a single microfluidic device. MCE is most coupled with laser‑induced fluorescence or universal conductivity detection [3]; however, expanding its applicability requires broader detection capabilities. Recent developments demonstrate successful coupling of MCE with UV–Vis detection [4], surface‑enhanced Raman spectroscopy [5], and ion mobility spectrometry [6], significantly extending its analytical reach.
Acknowledgements
The author gratefully acknowledges colleagues, Jasna Hradski, Peter Troška and Roman Szucs, for their valuable discussions, technical assistance, and continuous support. This work has been supported by the Slovak Research and Development Agency (APVV-22-0133), and the CEEPUS project RO-0010-2526: Teaching and Learning Bioanalysis.
References
[1] P. Troška, R. Chudoba, L. Danč, R. Bodor, M. Horčičiak, E. Tesařová, M. Masár, J. Chromatogr. B 2013, 930 41–47.
[2] M. Ďuriš, J. Hradski, R. Szucs, M. Masár, J. Chromatogr. A 2024, 1729, 465055.
[3] P. Troška, S. Dobosyová-Szalayová, R. Szucs, M. Masár, J. Food Compost. Anal. 2025, 137, 106905.
[4] M. Masár, J. Hradski, E. Vargová, A. Miškovčíková, P. Božek, J. Ševčík, R. Szucs, Separations 2020, 7, 72.
[5] M. Masár, P. Troška, J. Hradski, I. Talian, Microchim. Acta 2020, 187, 448.
[6] J. Hradski, M. Ďuriš, R. Szucs, L. Moravský, Š Matejčík, M. Masár, Molecules 2021, 26, 6094.